The distribution of glucocorticoid receptors (GR) in the rat testis was examined in vivo using (3H)-dexamethasone 21-mesylate (DM) which binds covalently to GR. After injection with $ sp3$H-DM, the testes of adrenalectomized, adult rats were processed for light microscope (LM) radioautographic (RAG) analysis. Control rats received simultaneously a 25-or 50- fold excess of cold dexamethasone. Quantitation of the label confirmed the presence of specific DM binding sites in both Leydig cells (86% of the label) and to a lesser degree, in the cellular layers of the seminiferous epithelium (14% of the label). These binding data were confirmed by LM immunocytochemistry. Interstitial macrophages were non-specifically labeled. In the Leydig cell, an electron microscope (EM) quantitative RAG analysis of 3H-DM binding sites showed that smooth endoplasmic reticulum (sER) and mitochondria were heavily labeled, with 53% and 31% of the total label, respectively. Cytosol (exclusive of all structures) and nucleus showed comparatively weak labeling, with 9% and 7% of the label, respectively. All other structures showed little or no labeling.
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59433 |
Date | January 1990 |
Creators | Stalker, Alison |
Publisher | McGill University |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Electronic Thesis or Dissertation |
Format | application/pdf |
Coverage | Master of Science (Department of Anatomy.) |
Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
Relation | alephsysno: 001233026, proquestno: AAIMM63623, Theses scanned by UMI/ProQuest. |
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