The aim of our investigation was to gain insight on the regulation of flagellar movement, at the axonemal level. In our laboratory a panel of monoclonal antibodies (MoAbs) has been produced against the axoneme of the biflagellated algae, Chlamydomonas reinhardtii, a well-characterized model for the study of flagellar movement. Of these MoAbs, L2H12 has been selected, because it has a potent inhibitory effect on the motility of de membranated-reactivated flagella of Chlamydomonas cells. Using video micrography, we demonstrated that low concentrations of L2H12 cause a progressive decrease in the wave amplitude and beat frequency of the flagella. Results of Western blotting of the axonemal proteins indicates that L2H12 recognizes a 105 kDa protein. Analysis of Chlamydomonas radial spoke mutants deficient in one or more radial spoke proteins (RSPs) suggests that this protein is RSP2. Immunoprecipitation of this protein was performed to further characterize it.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.31266 |
| Date | January 2000 |
| Creators | Magder, Ilana. |
| Contributors | Gagnon, Claude (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001802532, proquestno: MQ70462, Theses scanned by UMI/ProQuest. |
Page generated in 0.0018 seconds