In mice, H2 and Ly49h genes determine natural resistance to cytomegalovirus infection by alternate mechanisms. We are interested in MA/My, an inbred strain that is resistant to MCMV infection despite the absence of Ly49H receptor and the presence of a haplotype highly related to the MCMV-susceptible strains 129 and FVB/N. Therefore, it is interesting to study its genetic basis of resistance to MCMV infection. In this study, we have identified Cmv1rm, a new resistance allele conferring resistance in MA/My. We have demonstrated by statistical analyses that both H2 and NKC genes are important and that their genetic interaction is necessary to confer resistance in MA/My. By the characterization of the Ly49 gene repertoire of MA/My, we identified 3 potential activating Ly49 gene candidates. Finally, we have confirmed the presence of an important additive effect of H2 and NKC in a FVB/N x BALB.K cross, indicating that Cmv1 rm resistance mechanism may be present in other inbred strains.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.82222 |
| Date | January 2005 |
| Creators | Desrosiers, Marie-Pierre |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002198705, proquestno: AAIMR12433, Theses scanned by UMI/ProQuest. |
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