Genomic imprinting is a mechanism of fine regulation of gene expression. Imprinted genes are expressed from only one parental allele and many of them have critical roles in growth and development. Imprinting marks that distinguish the parental origin must be erased and re-established in germ cells according to the sex of the individual to ensure proper embryonic development. We investigated occurrence of imprinting errors in mouse embryos. Firstly, we tested the hypothesis that errors in resetting of imprints occur and lead to grandparental-origin effects on embryonic growth. Although we did not find statistically significant effects, we observed trends that should be confirmed by replication. Secondly, we examined expression of 5 genes located on the distal part of chromosome 12 in order to establish the incidence of spontaneous imprinting errors. We report a strain and parental-origin specific imprinting relaxation of Dlk1 and Dio3 genes.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.78337 |
| Date | January 2003 |
| Creators | Charron, Marie-Claude |
| Contributors | Naumova, A. K. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001975817, proquestno: AAIMQ88172, Theses scanned by UMI/ProQuest. |
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