The transcription factor FoxA2 and its closely related family member FoxA1 have been shown to both be expressed in the visceral and definitive endoderm, node, notochord and floor plate. Both factors have been shown to function as transcriptional activators and recognize the same DNA elements in vitro, suggesting that they may be functionally equivalent. However, there is increasing evidence, which suggest that they may play different roles in vivo. In order to begin to address whether FoxA1 is equivalent to FoxA2 in vivo, I used a gene targeting approach to replace the FoxA2 gene with the coding region of FoxA1, thus placing FoxA1 under the control of the FoxA2 regulatory elements. Screening of over 1600 clones allowed the identification of two correctly targeted ES cell lines. These ES cell lines will be an essential tool to address the functional equivalence of FoxA1 and FoxA2 in vivo.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.80303 |
| Date | January 2003 |
| Creators | Kim, Eujin, 1963- |
| Contributors | Dufort, Daniel (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002032074, proquestno: AAIMQ98672, Theses scanned by UMI/ProQuest. |
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