This thesis reports the identification and characterization of a novel gene, named 7/2, from soybean (Glycine max L. Merrill). Genomic clones of 7/2 from two different cultivars, cvs Maple Arrow and Resnik were isolated and sequenced, and the sequences were compared to each other. These sequences were 100% identical. Alignment with a cDNA sequence from cv. Maple Arrow revealed the structure of the gene as having 6 exons and 5 introns.
The full length cDNA contains an ORF of 798 by encoding a novel protein of 265 amino acids with a calculated molecular weight of 29.98 kDa. The conceptual 7/2 protein is similar in structure to Myb-like transcription factors in that it has an N-terminal DNA binding domain of 52 amino acid and a potential acidic activation domain. Unlike most known plant Myb transcription factors it has one, not two, N-terminal DNA binding repeats. As determined by RT-PCR, the7/2 message is expressed in most soybean tissues but is most highly expressed in nodules. These characteristics suggest that 7/2 may represent a novel soybean regulatory protein.
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/26969 |
| Date | January 2005 |
| Creators | Mansoorian, Neda |
| Publisher | University of Ottawa (Canada) |
| Source Sets | Université d’Ottawa |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 138 p. |
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