The gene designated Pom-1 is a new mouse gene identified in the region of the Gin-1 common proviral integration site. The origin of the Gin-1 region has been described elsewhere (Villemur et al., 1987). The novel Pom-1 gene is testis-specific and encodes two major RNA species of 1.0 and 1.2 kbp. Our data have revealed that there is no clear evidence to suggest that Pom-1 is involved in the tumorigenic pathway that resulted in the Gross Passage A Murine leukemia virus-induced thymomas. The pattern of expression of Pom-1 was studied by a time course Northern blot, the STAPUT cell separation technique and in situ hybridization. Analysis of mRNA from enriched populations of spermatogenic cells from adult testes and localization by in situ hybridization revealed that Pom-1 transcripts are most abundant in the round and elongated spermatids, although there is a weak expression in pachytene spermatocytes. Immunocytochemistry data have shown protein expression to be localized in the Golgi apparatus of pachytene spermatocytes (stages IX-XII), round spermatids (steps 5-8) and elongated spermatids (steps 9-15). / The Pom-1 gene is not homologous to any sequences present in the Genebank. Sequence analysis predicts a 6 kilodalton protein which is basic, lysine and arginine rich ($ sim$12%). It is also relatively rich in potential phosphoacceptor amino acids ($ sim$20%), mainly threonine and serine, several of which are located in phosphorylation consensus sequences. These results suggest a role for the novel Pom-1 gene in spermatogenesis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.34445 |
| Date | January 1996 |
| Creators | Schwartz, Rhonda L. (Rhonda Lynn) |
| Contributors | Jolicoeur, Paul (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001549448, proquestno: NQ30377, Theses scanned by UMI/ProQuest. |
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