We have studied spontaneous mutations in a chromosomally-located, single copy HSV-1 thymidine kinase (TK) gene in a human AK 143 TK$ sp-$ cell line. We have used three anti HSV-1 thymidine kinase nucleotide analogues, namely Trifluorothymidine (TFT), Acyclovir (ACV) and DHPG (9-(1,3-dihydroxy-2-propoxy-methyl)guanine), to select for $TK sp-$ mutants. The spontaneous mutation rate for the TK gene was very high in this system. The mutation frequency was 5 $ times$ 10$ sp{-3}$ per cell per generation when Acyclovir (ACV) was used as a selective agent and 5 $ times$ 10$ sp{-4}$ per cell per generation when Trifluorothymidine (TFT) was used. The spontaneous mutation frequency dropped to 1 $ times$ 10$ sp{-5}$ per cell per generation when a combination of TFT plus ACV was used and to 1 $ times$ 10$ sp{-6}$ per cell per generation when a combination of TFT plus DHPG (9-(1,3-dihydroxy-2-propoxy-methyl)guanine) was used as selective agents.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59622 |
| Date | January 1988 |
| Creators | Gupta, Kalpana |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Microbiology and Immunology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001167948, proquestno: AAIMM66345, Theses scanned by UMI/ProQuest. |
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