Salmonellosis is an important foodborne disease with world-wide economic and public health significance. Domestic poultry constitute one of the largest reservoirs of Salmonella in animal populations. Susceptibility to this infection varies in different chicken lines suggesting the involvement of host genetic factors. The gene, Nramp1, and the locus, Lps, are well-characterized in the mouse and are known to be involved in the early, innate responses to Salmonella infection. In our laboratory, we have used a comparative gene approach to examine whether NRAMP1 and LPS are important to chicken salmonellosis. / This thesis has continued our comparative examination through three objectives. The first objective was to determine the frequency of the NRAMP1 Arg223→Gln223 variant, originally found in Salmonella-susceptible chicken line C, in outbred chicken and pedigreed stock. Our second objective was to genetically map chicken TNC, a closely linked marker to LPS. A chicken backcross reference population was used to map this gene: the Compton mapping panel. We mapped TNC to microchromosome E41W17 in a region of conserved synteny with mouse chromosome 4 and human chromosome 9q. The third objective of this thesis was to develop chicken NRAMP1 antibodies in order to eventually characterize the protein's function and expression. The antisera raised in New Zealand White rabbits identified a 90--100 kDa species on a Western blot that most likely corresponds to the glycosylated chicken NRAMP1.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33478 |
| Date | January 2000 |
| Creators | Marshall, Jennifer. |
| Contributors | Malo, Danielle (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001753061, proquestno: MQ77064, Theses scanned by UMI/ProQuest. |
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