The goal of this project is to identify QTL involved in Salmonella -susceptibility in the chicken. Salmonellosis is one of the most common causes of food poisoning in humans and is frequently caused by the ingestion of Salmonella-contaminated poultry products. Identification of QTL responsible for Salmonella-susceptibility may lead to more efficient control strategies, and selection against genes that may lead to increased risk of transmission. The genome scan was performed on a selection of 425 backcross progeny, (W1 x C) F1 x C, derived from C Salmonella-susceptible and W1 Salmonella-resistant chickens. The 425 backcross birds were phenotyped for their susceptibility to infection with Salmonella Typhimurium. A collection of 160 fluorolabelled microsatellite markers (FAM, TET, and HEX) was used to scan the chicken genome, which is 3800cM in size (1.2 x 109 bp) and consists of 39 linkage groups, 9 chromosomes and 30 microchromosomes. We first tested the available microsatellite markers for informativeness in C and W1 chicken lines. In addition, we also determined the allele lengths and PCR product intensity (qualitatively) to facilitate pooling of markers during electrophoresis. PCR reaction mixtures were prepared using a Packard MultiProbe II Robotic System and Minitrak to pipette DNA-PCR Master mix and primer mix into microtiter plates. PCR products were then pooled and analyzed on Perkin-Elmer ABI Prism 3700 DNA Analyzers. Analysis of informative microsatellite markers on the backcross panel resulted in the detection of two loci, one on Chromosome 7 carrying NRAMP1, and the other on microchromosome E41W17 carrying TLR4, linked to resistance to Salmonella infection in chickens.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33758 |
| Date | January 2001 |
| Creators | Forgetta, Vincenzo. |
| Contributors | Malo, Danielle (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001862389, proquestno: MQ78875, Theses scanned by UMI/ProQuest. |
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