Normal and diseased human tissues were analyzed for the expression of genes of the carcinoembryonic (CEA) family. The use of portions of the CEA and NCA cDNA clones as hybridization probes has shown the existence of CEA and NCA transcripts in colon mucosa, NCA transcripts in breast tumours, and NCA and a third CEA-like transcript (CGM6) in chronic myelocytic leukemia (CML) cells. / A library prepared from CML cells was used to isolate a cDNA clone of CGM6. The predicted product of this novel CEA family member displays 85% sequence similarity to CEA and NCA and its expression is limited to CML and normal human bone marrow cells. / Southern blots of DNA did not reveal any CEA gene rearrangements or amplifications accompanying CEA expression. DNA digested with methylation-sensitive endonucleases demonstrated that CEA expression is correlated with a decreased level of methylation in the 5$ sp prime$ region of the CEA gene.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.39547 |
| Date | January 1992 |
| Creators | Boucher, Danny |
| Contributors | Fuks, Abraham (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001323924, proquestno: NN87694, Theses scanned by UMI/ProQuest. |
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