Adenovirus (Ad) infection is accompanied by an early inflammatory response that is stimulated by the binding and/or entry of the Ad capsid to the cell. Several studies have linked NF-kappaB to the activation of pro-inflammatory cytokines and chemokines early in Ad infection, however, for many other viruses, another key transcription factor, Interferon Regulatory Factor 3 (IRF-3), also acts early in viral infection. We sought to determine if IRF-3 is activated upon Ad infection, and to examine its importance in establishing an anti-viral state in Ad-infected cells. Our data suggests that wtAd5 infection results in phosphorylation of IRF-3 on a novel amino acid residue, and this event is dependent on virus replication. Moreover, IRF-3 is an important transcription factor for induction of RANTES expression in wtAd5-infected cells. Taken together, these data suggest that IRF-3 activation in response to Ad DNA replication is important in establishing an anti-viral state within the infected cell.
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/27453 |
| Date | January 2007 |
| Creators | Chaisson, Koralee |
| Publisher | University of Ottawa (Canada) |
| Source Sets | Université d’Ottawa |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 119 p. |
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