Laser preconditioning augments incisional wound healing by reducing scar tissue and increasing maximum tensile load of the healed wound. Under the hypothesis that HSP70 plays an active role in reported results and to better understand the downstream effects of laser preconditioning, this study utilized a probe-based Raman spectroscopy system to achieve an in-vivo, spatio-temporal biochemical profile of murine skin incisional wounds as a function of laser preconditioning and the presence of HSP70. Raman spectra yielded significant differences in known biochemical peaks between wild-type (WT) and HSP70 knockout mice. Analysis of peak ratios indicated 1) an increase in protein configuration on and surrounding the wound, and 2) increased cellularity that was prolonged in WT mice due to laser treatment. HSP70 is active in protein configuration and cellularity of early wound healing. Laser preconditioning enhances the effects of HSP70 in ways consistent with findings of previous studies. Raman spectroscopy proved a convenient non-invasive method of obtaining information for evaluating these effects and efficacy of wound healing laser treatment.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-04132010-093536 |
| Date | 16 April 2010 |
| Creators | Makowski, Alexander James |
| Contributors | Anita Mahadevan-Jansen, E. Duco Jansen |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-04132010-093536/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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