Both angiogenesis and inflammation are inescapable in vivo responses to any type of biomaterials implanted for regeneration. Continuous progress has been made in biomaterial design to facilitate tissue interactions with an implant by reducing inflammation and/or by inducing angiogenesis. However, it becomes increasingly clear that the physiological processes of angiogenesis and inflammation are interconnected through various molecular mechanisms. The role of implant-induced inflammation in the formation of new blood vessels into tissue surrounding the implant remains unclear. Therefore, we used a polyethylene glycol (PEG) cross-linked tyrosine derived polycarbonate hydrogel system as a model of implantable biomaterials. As opposed to the degradation rate, modulus and protein adsorption decreased as the cross-linking degree increased, due to hydrophilic repellent properties of PEG, indicating the unique and tunable hydrogel properties. The hydrogels were hybridized with pro- or anti-angiogenic (or inflammatory) peptides using collagen or fibrin gel and used for in vitro and in vivo biological studies. The results show a clear interconnectivity between angiogenic and inflammatory activities, indicating an inflammatory mechanism regulating follow-up angiogenic processes in hydrogels. This study suggests a new concept of biomaterial design that utilizes flexible inflammatory parameters to control angiogenesis for the eventual success of biomaterial implants.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-04042011-140317 |
| Date | 15 April 2011 |
| Creators | Zachman, Angela Laurie |
| Contributors | Scott Guelcher, Hak-Joon Sung |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-04042011-140317/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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