Given the prevalence of malaria in underdeveloped countries, specialized solutions must be developed for its diagnosis. The most common diagnostic throughout these regions is a lateral flow assay. While generally useful, these have a higher limit of detection than standard malaria diagnostics in the developed world. To help bridge this gap we are developing a system to concentrate malaria biomarkers on a lateral flow strip in order to lower its effective limit of detection. By using functionalized ferromagnetic micro-particles in a patient sample we can concentrate biomarkers and then transfer them to a surface leaving behind the bulk fluid. One of the first steps towards this is to understand parameters affecting particle transfer from an idealized fluid and across an air gap to a surface below. We were able to transfer various bead across distances of over 3mm for samples over 300?g when holding the sample orthogonal to the surface and over 200?g when holding the sample at a 45° angle to the surface. Bubble exchange between the seems to be the limiting factor driving bead transfer and occurs more readily in 45° samples allowing bead transfer to occur in an unimpeded fashion leading to more easily discernable trends within these samples.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-03222016-135821 |
| Date | 24 March 2016 |
| Creators | Baglia, Mark Louis |
| Contributors | Frederick R. Haselton, David W. Wright |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-03222016-135821/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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