To improve the surface properties of biomaterials, the effects of changes in surface chemistry and morphology of polyethylene terephthalate (PET) films treated with atmospheric pressure plasma were investigated as a function of cellular growth, proliferation, and adhesion. PET films were subjected to helium and helium/oxygen gas plasmas. The contact angle of the treated films decreased due to plasma etching and possible scission indicating that the surfaces become more hydrophilic. Atomic force microscopy results had a large standard error, however the surface visually showed changes in surface micro and nanoscale roughness corresponding to treatment duration. Keratinocytes were plated on the day of plasma treatment and two and five days after plasma treatment and tested half a day, one, two, three, and six days after plating. The same methodology of plating and testing was also applied to hepatocytes. Cell growth, proliferation, and adhesion were characterized via a fluorescent probe based assay and were correlated with surface chemical and nanostructural features. Both the helium and helium/oxygen plasma-treated PET had little or no effect on cell behavior for both keratinocytes and hepatocytes. The nanoscale surface changes due to the plasma surface treatment are believed to be masked by the protein adherence in the media on the surface of the PET.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-11022005-223628 |
| Date | 06 December 2005 |
| Creators | Christie, Megan Allison |
| Contributors | Mohamed Bourham, Wendy Krause, Hechmi Hamouda, Jeffrey Macdonald |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-11022005-223628/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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