The objective of this study was to image tumor-targeting using a single chain fragment variable antibody to radiation-induced P-selectin in a heterotopic Lewis lung carcinoma model by near-infrared fluorescence imaging and gamma camera imaging. In our in vitro studies, we observed antibody binding to P-selectin in endothelial cells treated with radiation. In our in vivo optical imaging study, it was observed that significant tumor-specific binding was observed in irradiated tumors expressing P-selectin as compared to unirradiated tumors. Gamma camera imaging also showed successful targeting to P-selectin in irradiated tumors with tumor binding lasting for up to 10 days post-injection. Therefore, radiation-induced P-selectin is a feasible target for the tumor-specific delivery of therapeutic drugs and radionuclides in vivo.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-04022007-233352 |
| Date | 14 April 2007 |
| Creators | Hariri, Ghazal |
| Contributors | Todd D. Giorgio, Dennis E. Hallahan |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-04022007-233352/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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