The processes of tumor growth and treatment response are associated with the upregulation of numerous proteins [1, 2], yet current clinical imaging methods of cancer characterization monitor only gross morphology [3, 4]. This study combines in vivo diffusion weighted magnetic resonance imaging (MRI) with matrix-assisted laser desorption ionization (MALDI) analysis of healthy and tumorous ex vivo specimens in order to examine the proteomic influences on the apparent diffusion coefficient provided by MRI. Spatial co-registration of MALDI and ADC datasets enables examination of the statistical correlations between the two metrics [5] in the hopes of elucidating the proteomic signatures that give rise to particular ADC values. <p>
ADC and MALDI data were acquired for two rats, one control and one in which a C6 glioma model of brain cancer was implanted. Principal component analysis was conducted to determine the degree of spatial correlation between the ADC and protein measurements. It was found that ADC and MALDI data correlated significantly (p-value of 0.05) in 44.0% of 114 regions of interest (ROIs) in the two rats. Protein profiles were identified which correlated with statistically similar ADCs in selected ROIs for each rat. The results of this study are consistent with the theory that protein expression in both healthy and tumorous rat brain tissue is a molecular-level source of contrast in diffusion-weighted MRI.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-03302009-151407 |
| Date | 07 April 2009 |
| Creators | Gillman, Amelie R. |
| Contributors | John C. Gore, Tuhin K. Sinha |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu//available/etd-03302009-151407/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
Page generated in 0.0016 seconds