The zebrafish is a robust model for studying human ophthalmic function and disease because of its fecundity, life-cycle, and similarities between its retinal structure and the human retina. Here, we demonstrate longitudinal in vivo imaging of retinal structure and, for the first time, noninvasive retinal vascular perfusion using optical coherence tomography (OCT) and OCT angiography (OCT-A) in zebrafish. In addition, we present methods for vascular segmentation and biometry to quantify retinal vessel length, branch angle, and curvature. We motivate retinal vascular biometry as a novel method for uniquely identifying zebrafish without the use of external markings and achieved 99.3% sensitivity and 99.9% specificity in a set of 200 longitudinal OCT/OCT-A datasets. The described methods enable quantitative analysis of vascular changes in zebrafish models of ophthalmic diseases and may broadly benefit large-scale zebrafish studies.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-04032018-144236 |
| Date | 12 April 2018 |
| Creators | Bozic, Ivan |
| Contributors | Yuankai Tao, Justin Baba |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-04032018-144236/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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