A theoretical and experimental method is described whereby the hydrodynamic forces, both normal and shear, acting on the spheres of a doublet can be calculated. This is applied to a system of sphered human red blood cells agglutinated by human hyperimmune anti-B antiserum undergoing Poiseuille flow and observed using the traveling microtube technique. The mean forces separating the cells of individual doublets were found to be proportional to antiserum concentration from 0.73 to 3.56% v/v, normal forces increasing from 0.060 to 0.197 nN and shear forces from 0.023 to 0.072 nN. It was impossible to determine which force was responsible for break-up. Measurements of the doublets' mean dimensionless period of rotation indicated that doublets were rigidly linked. / Micropipet aspiration was applied to the same red cell-antibody system. Separation forces were $ sim2{1 over2}$ fold greater than for normal forces of the traveling microtube technique. Non-uniformity of red cell adhesiveness was also demonstrated.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.75421 |
| Date | January 1987 |
| Creators | Tha, Susan P. L. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000419903, proquestno: AAINL38383, Theses scanned by UMI/ProQuest. |
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