This series of experiments systematically evaluates the effect of phospholipid headgroup structures on the interaction between rhodopsin and phospholipids. Three types of experiments are reported. / First, the effect of rhodopsin incorporation on the DMPC and DMPS gel-to-liquid-crystalline phase transition is analyzed with ESR techniques. Partial, binary phase diagrams of the DMPS- and DMPC-rhodopsin systems at pH = 7.0 are constructed by studying the partitioning of Tempo between polar and hydrophobic domains as a function of temperature and system composition. A main result of this analysis is that rhodopsin broadens and reduces the amplitude of the DMPS phase transition to a much smaller extent than the DMPC phase transition. When interpreted in terms of theoretical treatments of integral protein-lipid interactions, this indicates that rhodopsin has a lower affinity for DMPS than DMPC. / Second, ESR experiments involving nitroxide-labeled phosphatidylcholine, phosphatidylserine and phosphatidic acid are reported. / Third, the effect of the major headgroup classes of phospholipids on the conformational stability of rhodopsin is investigated. / This series of experiments demonstrate that the structure of the fatty acid chains is as important as the headgroup structure in determining the stabilization ability of a phospholipid. / Source: Dissertation Abstracts International, Volume: 42-09, Section: B, page: 3550. / Thesis (Ph.D.)--The Florida State University, 1981.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_74607 |
| Contributors | FISCHER, THOMAS HUGH., Florida State University |
| Source Sets | Florida State University |
| Detected Language | English |
| Type | Text |
| Format | 103 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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