Electric force microscopy has been adapted for fast, high-resolution imaging of the charge density of biological systems. The electric double layer interaction between the tip and sample provides a contrast mechanism that is sensitive only to surface charge density, as other parameters are held constant. This contrast is well described by an expression for the tip-sample double layer interaction in electrolyte solutions. The resultant charge maps are acquired at typical atomic force microscopy scan rates. Fluid electric force microscopy has proven highly sensitive and non-destructive, as demonstrated with charge density maps of fluid-phase supported bilayer membranes and single DNA molecules.
| Identifer | oai:union.ndltd.org:RICE/oai:scholarship.rice.edu:1911/17693 |
| Date | January 2004 |
| Creators | Johnson, Amber |
| Contributors | Hafner, Jason H. |
| Source Sets | Rice University |
| Language | English |
| Detected Language | English |
| Type | Thesis, Text |
| Format | 27 p., application/pdf |
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