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Process integration in the optimisation of amidase production from recombinant Escherichia coli

Includes abstract. / Includes bibliographical references (p. 127-138). / This thesis presents the investigation of the production of a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded bed adsorption (EBA) was assessed as a tool for minimising the unit operations in the amidase purification train. The EBA process can integrate up to four steps by merging processes involved in centrifugation, microfiltration and initial adsorption into one unit operation.

Identiferoai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uct/oai:localhost:11427/10857
Date January 2008
CreatorsOlaofe, Oluwafemi Ayokunle
ContributorsHarrison, STL
PublisherUniversity of Cape Town, Faculty of Engineering and the Built Environment, Centre for Bioprocess Engineering Research
Source SetsSouth African National ETD Portal
LanguageEnglish
Detected LanguageEnglish
TypeMaster Thesis, Masters, MSc
Formatapplication/pdf

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