Although there is information available on the distribution of
phospholipids in human serum and red cells, very limited data have
been reported for white cells and platelets. To the knowledge of the
author, no data have been reported on the distribution of phospholipids
among the four blood fractions, of individual subjects.
Due to the limited amounts of white cells and platelets present
in blood, micromethods are essential for the analyses of these fractions
in individual subjects. Although phospholipids have been separated
from larger samples by others, their methods were not appropriate
for micro amounts. Therefore, procedures were developed in
this study which made it possible to isolate and to quantitate the individual
components of samples of total phospholipid ranging from
20 to 40 μg.
The distributions of phospholipids were determined in serum,
red cells, white cells and platelets isolated from the venous blood
of four men and four women. The blood fractions were isolated and lipid was extracted by methods previously developed in this laboratory.
Total phospholipids were isolated by preparative thin-layer
chromatography. Individual phospholipid components were separated
and quantitated by the micromethod developed in this investigation.
Components were eluted from microchromatoplates and quantitated
by analysis of their phosphorus content. This method effectively
separated phospholipid samples into lysophosphatidylcholine (LPhC),
sphingomyelin (Sph), phosphatidylcholine + phosphatidylserine
(PhC + PhS) and phosphatidylethanolamine (PhE).
Although all of the fractions contained higher proportional
amounts of PhC + PhS than any other component, there were characteristic
distributions in each of the blood fractions. Serum was characterized
by high PhC + PhS and virtually no PhE. Red cells contained
lower amounts of PhC + PhS and more Sph and PhE than any
other blood fraction. The distributions of phospholipids in white cells
and platelets were similar and resembled the general pattern found
in red cells more than that of serum.
The marked differences in the distribution of phospholipids among
the blood fractions emphasize the importance of concurrent analyses
of all blood fractions in studies of human phospholipid metabolism. / Graduation date: 1966
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/26786 |
| Date | 14 May 1966 |
| Creators | Betshart, Antoinette Alice |
| Contributors | Hawthorne, Betty E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
Page generated in 0.0019 seconds