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Previous issue date: 2008-08-12 / Radiobiocomplexes are used in nuclear medicine to obtain images and to treat diseases. Blood constituents have been used as radiobiocomplexes. Natural or synthetic products
can influence on the labeling of blood constituents with technetium-99m (99mTc), the morphology of red blood cells and on the stannous chloride (SnCl2) action on plasmid
DNA. Sambucus australis and Sambucus nigra are used in popular culture for treating diseases. The aim of this work was to evaluate the effects of the extracts of Sambucus
australis and Sambucus nigra on the labeling of blood constituents with 99mTc, on morphology of red blood cells of Wistar rats, on the topology of plasmids DNA and the
action against the SnCl2 effects on the DNA of plasmids pBSK. On the labeling of blood constituents with 99mTc it was verified that both extracts were capable to decrease
significantly the radioactivity in the cellular compartment and in the insoluble fraction of plasma. Sambucus australis also decreased the labeling of insoluble fraction of blood cells
with 99mTc. Both extracts did not alter the morphology of red blood cells. Moreover, it was verified that Sambucus nigra did not alter the electrophoretic profile of plasmid DNA, but
decreased the effect of SnCl2 on plasmid DNA. These last results sugest a genotoxic effect and a protective action of Sambucus nigra extract against the SnCl2 action on
plasmid DNA. This work was developed with the contribution of several Departments of biomedical area of the Hospital Universit?rio Pedro Ernesto, of the UERJ, characterizing a
multidisciplinary experimental research / Radiobiocomplexos s?o utilizados em medicina nuclear para obten??o de imagens e tratamento de doen?as. Constituintes sangu?neos t?m sido utilizados como radiobiocomplexos. Produtos naturais ou sint?ticos podem influenciar a marca??o
de constituintes sang??neos com tecn?cio-99m (99mTc), a morfologia de hem?cias e a a??o do cloreto estanoso no DNA plasmidial. Sambucus australis e Sambucus nigra s?o utilizados na cultura popular para o tratamento de doen?as. O objetivo
desse estudo foi avaliar os efeitos dos extratos de Sambucus australis e de Sambucus nigra na marca??o de constituintes sang??neos com 99mTc, na morfologia de hem?cias de ratos Wistar, na topologia do DNA plasmidial e sua a??o sobre os
efeitos do cloreto estanoso no DNA de plasm?dios pBSK. Na marca??o de constituintes sang??neos com 99mTc verificou-se que os dois extratos foram capazes de diminuir significativamente a radioatividade no compartimento celular e na fra??o insol?vel do plasma. Sambucus australis diminuiu tamb?m a marca??o da fra??o insol?vel da c?lula com 99mTc. Os dois extratos n?o alteraram a morfologia das hem?cias. Al?m disso, foi verificado que um extrato de Sambucus nigra n?o alterou o perfil eletrofor?tico do DNA plasmidial, entretanto diminuiu os efeitos do cloreto estanoso no DNA plasmidial. Estes ?ltimos resultados sugerem um efeito
genot?xico e uma a??o protetora do extrato de Sambucus nigra contra a a??o do cloreto estanoso no DNA plasmidial. Este trabalho foi realizado com a colabora??o de diversos Departamentos da ?rea biom?dica do Hospital Universit?rio Pedro Ernesto, da UERJ, caracterizando uma pesquisa experimental multidisciplinar
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13234 |
| Date | 12 August 2008 |
| Creators | Ribeiro, Camila Godinho |
| Contributors | CPF:14967898491, http://lattes.cnpq.br/2204048396738679, Anselmo, Caroline Wanderley Souto Ferreira, CPF:97281204434, Caroline Wanderley Souto Ferreira Anselmo, Medeiros, Aldo da Cunha, CPF:07117701404, http://lattes.cnpq.br/9873289951810864, Catanho, Maria Teresa Jansem de Almeida |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias da Sa?de, UFRN, BR, Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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