The collection and preservation of llama semen is not a standardized procedure as it is in cattle. It has a number of complications that are mainly associated with the characteristics of the semen. In order to contribute to the knowledge of reproductive biology, we decided to evaluate the behavior of llama semen in relation to the different diluters in this study. This study was done at the National Germplasm Bank for Camelids which is part of the Ministry of Rural Development of Agriculture and Environment. The Ministry is located in the Agriculture Experiment Center of Condoriri which belongs to the College of Agriculture, Livestock, and Veterinary Sciences of the Technical University of Oruro. The University is located 49 km northeast of the province of Cercado in the department of Oruro and 12 km northeast of the community of Caracollo. The geographic coordinates of Caracollo are 17º31’41” south latitude and 67º14’02” west longitude. It is at an altitude of 3830 m above sea level and has an area of 1640 ha (Geographical map of the Military Geographical Institute). The macroscopic evaluations made were: pH (7.2 - 7.6), volume (2 - 4.5 cc), color (clear white or milky white), density, or consistency by simple inspection (subjective). The microscopic evaluations made were: motility (50 - 70%), viability (50 - 70%), concentration (20 - 40 x 107/esp./cc), and abnormalities (10% at most). All materials and environments used in this assessment were at room temperature. The analysis of variance test at 99% probability showed that the macroscopic and microscopic characteristics are affected by different harvest times. The two diluter preparations studied were 1) PBS diluter (with all its components that will serve the sperm as energy, nutrient, and cushioning), egg yolk, blood serum, and glycerol, and 2) TRIS diluter (with all its components that will serve the sperm as energy, nutrient, cushioning, and elements to avoid contamination), egg yolk, blood serum, and glycerol. All these materials were brought to 35 - 37ºC before being added to the semen. The prepared samples containing the diluent were then cooled to 5ºC. The samples were then collected for storage, with special care to maintain the sample temperature. The freezing was done slowly by placing the rack of containers at a given height of liquid nitrogen for seven to ten minutes. Following this, the containers were submerged. This slow freezing process prevented problems in the metabolism of the sperm after thawing. The samples were unthawed at intervals of 15 and 30 days by rapidly removing them from the liquid nitrogen and placing them in a water bath for 30 seconds. The evaluations of viability, motility, and concentration were all performed within 15 minutes of thawing. The results obtained from the analyses after thawing on average were better for the diluter PBS than for the diluter TRIS. The analysis of variance test at 99% probability showed that the characteristics after thawing were affected differently according to the diluter used in the dilution.
| Identifer | oai:union.ndltd.org:BGMYU2/oai:scholarsarchive.byu.edu:etd-6336 |
| Date | 01 January 2007 |
| Creators | Bustos Fernández, Franz Nicolas |
| Publisher | BYU ScholarsArchive |
| Source Sets | Brigham Young University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
| Coverage | Oruro (Bolivia) |
| Rights | http://lib.byu.edu/about/copyright/ |
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