BACKGROUND: Alveolar bone resorption is more severe in type 2 diabetes-potentiated periodontitis. The key cells responsible for bone resorption are the osteoclast and their quantity and activity are regulated by the RANK/RANKL/OPG system. One of the major RANKL-expressing sources in diseased periodontal tissue are activated B and T lymphocytes, therefore it was hypothesized that type 2 diabetes up-regulates B cell RANKL function.
OBJECTIVE: The aim of this study was to compare the effect of diabetes on the ability of B and T lymphocytes to stimulate osteoclast activity.
MATERIALS AND METHODS: Metabolic status (diabetic vs normal) of mice were established using either a low-fat diet (LFD) or high-fat diet (HFD). Spleen lymphocytes were harvested and purified, then cultured in different combinations with adherent mouse leukemic monocyte/macrophage cell line cells over dentin-coated wells and observed for osteoclastic activity that breaks down the dentin-coating. The area of clearance was used to represent the level of activity.
RESULTS: No difference in osteoclastic activity were noted between the two metabolic statuses, between B or T cells, between lymphocyte stimulated or not.
CONCLUSION: The findings counters our hypothesis, and are inconsistent with the currently available evidence. Repeat of the experiment is warranted before valid conclusion can be drawn from the data.
| Identifer | oai:union.ndltd.org:bu.edu/oai:open.bu.edu:2144/37068 |
| Date | 16 July 2019 |
| Creators | Chiu, Kai-Jen Jerry |
| Contributors | Nikolajczyk, Barbara S. |
| Source Sets | Boston University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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