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Amperomotric detection of sulfur-containing amino acids by capillary electrophoresis using boron-doped diamond microelectrode

The fabrication and characterization of boron-doped diamond
microelectrodes for use in electrochemical detection
coupled with capillary electrophoresis (CE-EC) is
discussed. They exhibited low and stable background
currents and sigmoidally shaped voltammetric
curves for cysteine, cystine and Fe(CN)63-/4- .
Evaluation of the CE-EC
system and the electrode performance were accomplished
using a 10 mM borate buffer, pH 8.8, run
buffer, and a 70-cm-long fused-silica capillary (10-mm
i.d.) with seven sulfur-containing amino acids
(methionine, cysteine, cystine, homocysyeine, homocystine,
glutathionine, glutathionine disulfide) as test analytes.
Reproducible separation (elution time) and detection (peak
current) of seven sulfur-containing amino acids were observed with response precisions of 5% or less.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0802104-153250
Date02 August 2004
CreatorsLiu, Jung-chung
ContributorsShiuh-Jen Jiang, Tai-Fa Young, Tai-Sung Hsi
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802104-153250
Rightswithheld, Copyright information available at source archive

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