This thesis is based on the use of ISSR method for studying genetic diversity of oilseed rape. Altogether, 180 genotypes were evaluated. Frozen leaves of oilseed rape from three breeding station called: 1/ breeding station Selgen a.s., 2/ OSEVA PRO s.r.o. and 3/ breeding station Slapy u Tábora were used as imput material. DNA isolation was performed by Williams, who modified CTAB method for oilseed rape. We selected for ISSR 4 UBC which showed high variability and stability. The analysis was performed by gel electrophoresis. This thesis is also focused on comparison of gel electrophoresis and chip electrophoresis. In this thesis We show a matrix of simmilarity between varieties. The results were also provided back to breeders as a basis for further breeding.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:376222 |
| Date | January 2018 |
| Creators | ČÍŽKOVÁ, Pavla |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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