Members of the Dlg/PSD-95 protein family have a modular organization with multiple protein interaction domains and are thought to be important in the organization of synapses. A degenerate primer PCR strategy was used to screen for members of this family in the central nervous system of Apteronotus leptorhynchus, which possesses an electrosensory system that is well-suited for the study of synaptic organization and the subcellular localization of proteins. A. leptorhynchus was found to express at least four Dlg/PSD-95 family genes as in mammals, and the full coding sequences of the homologues of mammalian PSD-95 and SAP102 were determined. In situ hybridization experiments performed for the A. leptorhynchus Dig/PSD-95 family members in brain showed that they have a differential pattern of expression.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.29906 |
| Date | January 1999 |
| Creators | Lee, Sang, 1972- |
| Contributors | Dunn, Robert J. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001687366, proquestno: MQ55074, Theses scanned by UMI/ProQuest. |
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