The roles of vitamin C and the effects of supplementation have been under investigation for many years. The purpose of this study was to use high performance liquid chromatography to assess the presence of vitamin C in human sweat, to evaluate sweat as a possible excretory route for vitamin C and to note any effects of vitamin C supplementation. Vitamin C in sweat was determined in samples from 10 active men and women prior to supplementation and weekly for 4 consecutive weeks as the vitamin C supplements were with a reverse-phase liquid chromatograph (Model ALC-202) equipped with a solvent delivery system (Model 6000, Waters Association).The presence of vitamin C in sweat samples was indicated by comparing peaks to those seen with vitamin C observed in sweat samples. Vitamin C was observed in sweat at the end of the first week of supplementation. The pres C.However, 4 and 5 when standards. During the first week of the study, when subjects' diets were not supplemented, vitamin C was not observed in sweat samples. Vitamin C was observed in sweat at the end of the first week o supplementation. The presence of vitamin C in sweat diminished during weeks 4 and 5 when intakes were supplemented with 750 mg and 1000 mg, respectively. It appeared that supplementation affected the presence of vitamin C in human sweat until plasma or tissues were saturated, at which point absorption may have decreased and other means of excretion may have been enhanced.
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/183038 |
| Date | 03 June 2011 |
| Creators | Davisson, Corine Mary Little |
| Contributors | Roepke, Judith B. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | v, 60 leaves ; 28 cm. |
| Source | Virtual Press |
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