Campylobacter is an important human pathogen that colonizes the gut of food
producing animals. In this study, the effects of 2-nitro-1-propanol, 2-nitroethanol,
nitroethane, and 2-nitro-methyl-propionate (0, 10, and 20 mM) on growth of
Campylobacter jejuni were tested during culture in Bolton Broth adjusted to pH 5.6, 7.0,
or 8.2. The effects of the nitrocompounds were also tested against C. coli in Bolton
Broth but adjusted to pH 8.2 only. Viable cell counts of samples taken at intervals during
incubation revealed main effects (P < 0.05) of nitroethane, 2-nitro-1-propanol, 2-
nitroethanol, and 2-nitro-methyl-propionate as evidence by reduced survivability of C.
jejuni. A marked effect of pH on the survivability of C. jejuni during incubation with all
compounds was observed, with greater activity observed at pH 8.2 than at pH 5.6 or 7.0
for nitroethane, 2-nitro-1-propanol, 2-nitroethanol, but not for 2-nitro-methyl-propionate.
In the case of 2-nitro-methyl-propionate, survivability of C. jejuni was reduced most at
pH 5.6. Except for 2-nitro-methyl-propionate, which was ineffective, all nitrocompounds
elicited similar effects on C. coli when cultured at pH 8.2. The effect of nitroethane and
2-nitro-1-propanol (10 mM) on naturally-occurring Campylobacter was further
investigated during incubation of a porcine fecal suspension. Campylobacter concentrations decreased more rapidly (P < 0.05) during incubation of porcine fecal
suspensions supplemented with 2-nitro-1-propanol than unsupplemented control
suspensions or suspensions supplemented with nitroethane thus reiterating the superior
inhibitory effect of 2-nitro-1-propanol.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/5022 |
| Date | 25 April 2007 |
| Creators | Horrocks, Shane Michael |
| Contributors | Anderson, Robin C., Ricke, Steven C. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | 346035 bytes, electronic, application/pdf, born digital |
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