Project objectives were to develop an improved plating medium to achieve reproducible colony counts and accurate quantification for growth or survival of Spirillum volutans; and to develop an improved method for selection of S. volutans from nature.
Growth enhancement studies began with casein hydrolysate-succinate-salts medium (CHSS). Casein hydrolysate concentration, type of casein hydrolysate, pH, and phosphate concentration optima were determined. Growth of S. volutans doubled after 24 h with the addition of potassium phosphate buffer (1 mM final concentration) to CHSS medium (CHSS-P). Addition of supplements to CHSS-P medium, and modified Bordetella and Brucella media failed to increase the cell yields of S. volutans. Punctiform colonies did form in CHSS-P medium (0.7% agar) pour-plates, yet at a recovery rate much lower than reported by Padgett et at. (1982) for surface growth on spread plates. The role of amino compounds as possible growth factors was investigated. Thin-layer chromatography, gas chromatography, and amino acid analysis were employed to identify those amino compounds (if any) that decreased or disappeared in the medium after 48 h of growth. None could be found. Numbers of viable cells were not greatly increased, however, viability was prolonged by growing S. volutans in dialysis sacs suspended in CHSS-P medium.
The tolerances of S. volutans to several antimicrobial compounds were determined. Attempts at using hay infusion, Pringsheim, and CHSS-P media supplemented with various compounds as selective agents did not result in predominance of S. volutans. / M.S.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/106185 |
| Date | January 1987 |
| Creators | Friedman, Michael W. |
| Contributors | Microbiology |
| Publisher | Virginia Polytechnic Institute and State University |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis, Text |
| Format | xiii, 106 leaves, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 17374758 |
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