Bird¡¦s nest is the saliva from the Colloclia that have a lot of mucin and sialic acid. However the sialic acid is an important intermediate of influenza virus infection. In 2006, Guo et al was confirmed the Inhibitory effect of bird¡¦s nest extract on influenza virus infection. But the anti-virus component has not been isolated and purified. First, the bird's nest used in this study was proved to be a pure bird¡¦s nest by SDS-PAGE, sialic acid content and protein content. Qualification analysis by SDS-PAGE showed sample possessed three bands at about 50 kDa, 100 kDa and 150 kDa, by Warren assay showed sample possessed a sialic acid content about 10% of dry weight, by Kjeldahl method showed sample possessed a protein content about 50% of dry weight, and by fluorescence analysis in 365 nm showed sample emitted a blue-white light. According to the criteria on previous studies, the sample was considered to be a real and pure bird¡¦s nest. Subsequently, the sample was applied to pronase digestion and then separated by Bio-gel P2 for size exclusion chromatography. All fractions were performed carbohydrate analysis including sialic acid. In lectin blotting, Maackia amurensis agglutinin (MAA) lectin can interact with the fraction that suggest the structure of sialic acid is sialic acid-2,3 galactose in bird¡¦s nest. And carbohydrate membrane array to demonstrate the influenza virus of H5N1 and H1N1 can bind the fractions that suggest the antivirus effect of bird¡¦s nest. These fractions will be further analyzed by neutralization assay to study their antivirus effects in vitro.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0812111-022949 |
| Date | 12 August 2011 |
| Creators | Chen, I-Jen |
| Contributors | Kuang- hung Cheng, David Chao, Chuan-Fa Chang, Tzann-Shun Hwang, Li-Wen Fang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0812111-022949 |
| Rights | user_define, Copyright information available at source archive |
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