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Previous issue date: 2014-03-26 / Biomphalaria snails act as intermediate hosts that harbor the Schistosoma mansoni parasite, the causative agent of schistosomiasis. As population increases, snails have their geographical distribution conditioned to the presence of calcium in the environment, which is essential for their energetic metabolism. The objective of this study was the assessment on the influence of exposure to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) at different intervals (1, 14, 21 and 30 days) forthe concentrations of glucose, calcium, total proteins, urea, uric acid and creatinine in hemolymph B. glabrata. All substances were dosed using specific kits with a spectrophotometer reading at 545 nm. High performance liquid chromatography (HPLC) was the technique applied to quantify the presence of organic acids: pyruvate, oxaloacetate, citrate, succinate, fumarate, propionate, acetoacetate and βhydroxybutyrate in the hemolymph of mollusks exposed to CaCO3. Results revealed
that the calcium concentration in the hemolymph did not present significant difference
(p>0.05) with regard to control as well as to the concentrations assessed. The glucose
concentration decreased (p<0.05) in exposures to 20mg and 40mg and increased in
exposures to 80mg and 100mg of CaCO3 with regard to control. The organic acids
pyruvate, oxaloacetate, citrate, succinate fumarate and lactate had their concentrations
increased; propionate in turn had its concentration decreased set against control in
exposure to CaCO3. In the exposures to the concentrations assessed, acetoacetate
decreased and β-hidroxibutirato increased. Total proteins and urea presented decrease.
The uric acid concentration increased in the exposure to 20mg of CaCO3; the creatinine
concentration increased in the exposures to 40mg and 100mg of CaCO3. It was possible
to conclude that snails exposed to different concentrations of CaCO3 had their major
metabolic alterations occurring from the 14th day of exposure. / Moluscos do gênero Biomphalaria são conhecidos pelo seu papel como hospedeiro intermediário do parasito Schistosoma mansoni, causador da doença esquistossomose mansônica. O aumento da população desses moluscos, consequentemente sua distribuição geográfica está condicionada a presença de cálcio no mbiente, também considerado um elemento essencial no metabolismo energético do molusco. Este estudo teve por objetivo avaliar a influência da exposição às diferentes concentrações de CaCO3 (20, 40, 60, 80 e 100 mg/L), em diferentes intervalos de tempo (1, 14, 21 e 30 dias) sobre às concentrações de glicose, cálcio, proteínas totais, ureia, ácido úrico e creatinina na hemolinfa de B. glabrata, ambas as substâncias foram dosadas através de kits específicos e lidas em espectrofotômetro em 545 nm. Através da técnica de cromatografia líquida de alta eficiência (CLAE), foi quantificado a presença de ácidos orgânicos: piruvato, oxaloacetato, citrato, succinato, fumarato, propionato, acetoacetato e β-hidroxibutirato na hemolinfa dos moluscos expostos ao CaCO3. Os resultados demonstraram que a concentração de cálcio na hemolinfa não apresentou diferença
significativa (p>0,05) em relação ao controle, bem como, entre as concentrações testadas. A concentração de glicose diminuiu (p<0,05) nas exposições a 20mg e 40mg e aumentou nas exposições a 80mg e 100mg de CaCO3 em relação ao controle. Os ácidos orgânicos piruvato, oxaloacetato, citrato, succinato, fumarato e lactato tiveram suas concentrações aumentadas e propionato diminuída comparada ao controle na exposição
ao CaCO3. A concentração de acetoacetato diminuiu e a de β-hidroxibutirato aumentou na exposição às concentrações testadas. Houve diminuição na concentração de proteínas totais e de ureia. A concentração de ácido úrico aumentou na exposição a 20mg de CaCO3 e, a concentração de creatinina aumentou nas exposições à 40mg e 100mg de CaCO3. Concluiu-se que moluscos expostos às diferentes concentrações de CaCO3, principalmente a partir de 14 dias, alteram o metabolismo como acontece em situações de estresse fisiológico, com a finalidade de regular o sistema redox do molusco.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.bc.ufg.br:tede/3111 |
| Date | 26 March 2014 |
| Creators | Silva, Luciana Damacena |
| Contributors | Bezerra, José Clecildo Barreto, Castro, Ana Maria de, Mello-Silva, Clélia Christina Corrê de, Bezerra, José Clecildo Barreto, Castro, Ana maria de, Mello-Silva, Clélia Christina Corrêa de, Vinaud, Mariana Clare, Barcelos, Rejane da Silva Sena |
| Publisher | Universidade Federal de Goiás, Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP), UFG, Brasil, Faculdade de Medicina - FM (RG) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFG, instname:Universidade Federal de Goiás, instacron:UFG |
| Rights | http://creativecommons.org/licenses/by-nc-nd/4.0/, info:eu-repo/semantics/openAccess |
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