The effects of buffer additives in capillary electrophoresis (CE) separations of various biologically important molecules were studied. Buffers containing $ beta$-cyclodextrin ($ beta$-CD) and borate were used to separate mixtures of monophosphorylated nucleotide isomers. The electrophoretic mobility of nucleotide isomers was altered as a result of selective complex-forming reactions with $ beta$-CD. In addition, complexation of 5$ sp prime$-nucleotides with borate increased the migration time window and led to better separation. / Mono- and di-phosphorylated isomers of the insulin receptor peptide (IRP), were resolved in buffers containing sodium dodecyl sulfate, (SDS). The effects of SDS concentration and pH on electrophoretic velocity and resolution were investigated. The use of polyacrylamide coated capillaries resulted in increased resolution and decreased analysis time. / The effects of various detergents on the electrophoretic behaviour of plasma apolipoproteins in uncoated and polyacrylamide coated capillaries were studied. Interaction between apolipoproteins and detergents was strongly influenced by the nature of the polar head group of the detergent. / Capillaries coated with various crosslinked hydrophillic polymers were prepared. Coatings based on poly(2-hydroxyethylmethacrylate) crosslinked with dimethylol ethylene urea exhibited the best performance for CE separations of basic proteins.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.39880 |
| Date | January 1995 |
| Creators | Tadey, Tanya |
| Contributors | Purdy, William C. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001476597, proquestno: NN08160, Theses scanned by UMI/ProQuest. |
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