Two new techniques for the study of biological molecules using vibrational spectroscopy have been investigated, and are presented in this thesis in two parts. / In Part I, the application of centrifugal vacuum techniques for sample preparation in FT-IR spectroscopy is demonstrated. This technique is used in the development of an agglutination immunoassay for bovine serum albumin (BSA) using polystyrene latex particles as both a solid support and an IR label. The synthesis of new organometallic labels for use in LPIRIA has been undertaken and is described in detail. A competitive non-agglutination LPIRIA for BSA using anti-BSA covalently bound to carboxylate-modified latex (CML) particles has been developed. Protein-A, a universal antibody binder, covalently bound onto CML, is used in the development of a competitive non-agglutination LPIRIA for nortriptyline and for the titration of anti-theophylline by theophylline. / In Part II, the study of a variety of ($ eta sp{6}$-arene) chromium tricarbonyl complexes and cyclopentadienyl manganese tricarbonyl by FT-Raman spectroscopy is reported. The application of FT-Raman to the study of proteins, mycotoxins, latex particles, and selected drugs is also presented. (Abstract shortened by UMI).
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59566 |
| Date | January 1990 |
| Creators | Barnett, Steven Marc |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001073574, proquestno: AAIMM63716, Theses scanned by UMI/ProQuest. |
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