The role of mobile phase modifiers in supercritical fluid chromatography (SFC) and their effect on the separation process were investigated. A study of the influence of temperature and density on chromatographic behaviour in supercritical carbon dioxide and four modified carbon dioxide mobile phases demonstrates the importance of both density and mobile phase composition in controlling retention characteristics. The information gained from these studies was used to develop methodology for the separation and detection of a series of phenothiazinone compounds and an assay for L-615,919, 4-chloro-3H-phenothiazin-3-one, in plasma. The development of an analytical method for the analysis of sorbitan trioleate in pharmaceutical formulations demonstrates a unique application of SFC with flame ionization detection (FID) that is not possible by other chromatographic techniques. / The feasibility of an electrochemical detection system for SFC has been demonstrated. The design and construction of an electrochemical detector with a platinum ultramicro working electrode and factors influencing its performance are described.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74532 |
| Date | January 1990 |
| Creators | Di Maso, Marie |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001171590, proquestno: AAINN66359, Theses scanned by UMI/ProQuest. |
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