Phosphorus magnetic resonance spectroscopy (31P-MRS) permits the measurement of high-energy phosphates in the gastrocnemius muscle at rest and during recovery from exercise. This examination is a powerful non-invasive method to evaluate muscle mitochondrial function in vivo. Data analysis, however, can be both time-consuming and user-dependent. I designed a fully-automated system to quantify both resting and recovery spectra, followed by quantification of metabolite recovery kinetics. This included quantifying the recovery kinetics of adenosine diphosphate (ADP), an index of mitochondrial function which previously had not been properly characterized. I modeled the transition from ischemic-exercise to perfused-recovery as a step function, and fitted the ADP recovery with a second-order step-response function. Furthermore, I used nearest-neighbour methods to account for the effects of physical conditioning and metabolic work on the recovery kinetics. This fully-automated method of analyzing 31P-MRS data provides comprehensive results relevant to the diagnosis and monitoring of patients with metabolic myopathies.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.30237 |
| Date | January 1999 |
| Creators | Chen, Jacqueline T., 1973- |
| Contributors | Arnold, D. L. (advisor), Kearney, R. E. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biomedical Engineering.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001740668, proquestno: MQ64213, Theses scanned by UMI/ProQuest. |
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