Derivatized $ beta$-cyclodextrin was used as mobile phase additive for the high performance liquid chromatographic resolution of amino acid derivative enantiomers while using an achiral C$ sb{18}$ stationary phase. Dimethyl-$ beta$-cyclodextrin gave improved enantioselectivity over the underivatized $ beta$-cyclodextrin. The effects of cyclodextrin concentration, pH, and methanol and buffer concentration on enantioselectivity and capacity factor were examined. / $ beta$-Cyclodextrin and two derivatized cyclodextrins were utilized as mobile phase additives for the liquid chromatographic resolution of equilin and estrone as well as estrone from 2-, 4-, and 16$ alpha$-hydroxyestrone. $ beta$-cyclodextrin proved to be suitable in these separations but the modified $ beta$-cyclodextrins provided better resolution. Apparent inclusion complex strengths were calculated for estrone and its derivatives were calculated for each cyclodextrin. / Micellar electrokinetic chromatography using sodium dodecyl sulphate with $ beta$-cyclodextrins was found to give comparable or improved separation of a series of fat-soluble vitamins over reversed-phase high performance liquid chromatographic techniques with cyclodextrins in the mobile phase. / Charged carboxymethyl-$ beta$-cyclodextrin was used in the capillary electrophoretic separation of a series of nine tricyclic antidepressants. Cyclodextrin alone was successful in separating some of the compounds under investigation while complete separation required the addition of a micellar pseudophase. A variety of coatings were investigated to reduce or eliminate the electroosmotic flow.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.40259 |
| Date | January 1996 |
| Creators | Spencer, Brian, 1967- |
| Contributors | Purdy, William C. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001498821, proquestno: NN12489, Theses scanned by UMI/ProQuest. |
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