The physical properties and renaturation kinetics of DNA extracted from isolated chloroplasts of Acetabulavia meditewanea has been studied. 3 It has a buoyant density of 1.702 g/cm³ , which corresponds to a base composition
of 42.8% G+C. When melted in SSC, Acetabulavia chloroplast DNA has a Tm of 86.7°, corresponding to a base composition of 43% G+C. The close agreement of the base compositions calculated from the buoyant density
and the melting temperature indicates the absence of unusual bases in Acetdbularia meditewanea chloroplast DNA. In 0.1 x SSC, Acetabulavia chloroplast DNA melts with a Tm of 70.7°, and the melting transition is very broad. The breadth of the melting transition
suggests that this DNA has a high degree of intramolecular heterogeneity.
A differential plot of the thermal transition of A. meditewanea chloroplast DNA supports this conclusion. The buoyant densities of DNA from bacterial contaminants found in Acetabulavia cultures differed from the buoyant density of the chloroplast DNA. In any case, the amount of bacterial contamination was too low to account for any of the results obtained. Renaturation experiments indicate a kinetic complexity of 1.1 x 10⁹ daltons from Acetabulavia meditewanea chloroplast DNA. As a result of uncertainties
in the values of alkaline sedimentation coefficients, this calculated
kinetic complexity may be too low. The possible genetic information contained in the chloroplast DNA of Acetabulavia meditewanea is discussed. / Science, Faculty of / Botany, Department of / Graduate
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/18900 |
| Date | January 1974 |
| Creators | Muir, Bernice L. |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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