The purpose of this research was to investigate selected methods of mucosal
immunization for commercial chickens. Induction of mucosal immunity in commercial
chickens through the use of orally administered subunit vaccines or through
immunomodulation of the host??s response to live vaccines may be a viable means to
control enteric infections in commercial poultry. In the present investigations we
evaluated a means for delivering protein antigen in the drinking water and the use of
CpG-ODNs, a recently reported mucosal adjuvant, in order to both improve this
response and to modulate the host??s immune response when vaccinated with field strains
of Eimeria acervulina and Eimeria tenella.
In order to evaluate the efficacy of immunizing commercial poultry with subunit
vaccines through the drinking water we chose the model antigen Bovine Serum Albumin
(BSA). Chicks were administered BSA via intraperitoneal (I.P.) injection, oral crop
gavage, or orally through the addition of BSA to the drinking water. These experiments
demonstrated the efficacy of drinking water administration to induce antibodyproduction in the serum, intestine, and bile. When BSA was co-administered with CpGODNs
we observed a modest increase in this response dependent upon dose.
To evaluate the immunomodulation of the host response to live parasite using
CpG-ODNs we used three administration models. The first was a single dose of CpGODNs
with a trickle immunization regime of Eimeria acervulina. The second was coadministration
of CpG-ODNs with a clinical dose of Eimeria acervulina or tenella. The
third was pre-administration of CpG-ODNs 24 hours prior to the clinical dose of either
species. These studies demonstrate that the first and third models were effective in
reducing lesions and improving performance.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/2421 |
| Date | 29 August 2005 |
| Creators | Ameiss, Keith Allen |
| Contributors | Caldwell, David J. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation, text |
| Format | 418066 bytes, electronic, application/pdf, born digital |
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