Several classes of genes determine the lifespan of the nematode Caenorhabditis elegans. Our laboratory is particularly interested in the clk class of genes that is composed of clk-1, clk-2, clk-3 , and gro-1. Mutations in these genes have been shown to extend lifespan and to deregulate several developmental and behavioural processes such as pharyngeal pumping and defecation cycle length. / clk-1 encodes a 187 amino acid mitochondrial protein that is composed of two homologous TRC domains (TRC for T&barbelow;andemly R&barbelow;epeated in C&barbelow;LK-1). Interestingly, the yeast homologue of clk-1, COQ7, has been implicated in ubiquinone biosynthesis. clk-1 (e2519) lesion is a point mutation that changes a conserved amino acid (E148K) in the second TRC domain. A structural model proposed that clk-1 is a di-iron carboxylate protein. We found that mutations in the di-iron binding center abolish CLK-1 activity and modify the subcellular distribution of CLK-1 in clk-1( qm30) null mutants.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33853 |
| Date | January 2001 |
| Creators | Ubach, Antonio. |
| Contributors | Hekimi, Siegfried (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001872096, proquestno: MQ78970, Theses scanned by UMI/ProQuest. |
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