To understand the molecular basis of developmental control of cell division during C. elegans organogenesis, two different approaches were taken. First, a screen was performed to identify mutants with altered numbers of intestinal nuclei using a reporter transgene specific to the intestinal nuclei. The intestine displays three different cell division patterns; mitosis, karyokinesis and endoreplication, therefore, in this screen we could potentially isolate mutants in genes affecting any of these different cell cycles. An F2 semi-clonal screen was performed and mutants with fewer or supernumerary numbers of intestinal nuclei were isolated. One mutant, rr31, with twice the wild type complement of intestinal nuclei was mapped and the defect was subsequently shown to be due to a gain-of-function mutation in the cell cycle phosphatase cdc-25.1. Further characterization of the cdc-25.1(gf) mutant, showed that the extra intestinal cells arise from an additional division of the intestinal cell precursors during embryogenesis, and that this phenotype is unique to the intestinal lineage. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.29448 |
| Date | January 2002 |
| Creators | Kostić, Ivana |
| Contributors | Roy, Richard (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001955804, proquestno: MQ85799, Theses scanned by UMI/ProQuest. |
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