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Previous issue date: 2016 / O objetivo desse trabalho foi avaliar os efeitos da efedrina no comportamento de ratos tratados com ra??o padr?o (comercial), dieta de cafeteria ou dieta de restri??o cal?rica desde a lacta??o. Foramutilizadas 12 ninhadas de ratos da linhagem Wistar (Rattus novergicus). Os animais foram alojados em gaiolas individuais, sob condi??es padr?es (umidade natural; temperatura de 23 ?C ? 2; e ciclo claro/escuro de 12 horas). Os ratos machos de cada ninhada formaram, da lacta??o at? a fase adulta, osgrupos: Controle (C) ?receberamra??o padr?o e?guaadlibitum(n = 24);Cafeteria (CAF)?receberam dieta de cafeteria e ?gua ad libitum (n = 24); Restri??o (R) ?receberam 50%dara??oconsumidapelo grupoControle e ?guaadlibitum (n = 24). Entre o 113? e o 117? dia de vida, os animais foram subdividos (n = 12) para receberemo tratamento com salina (C, CAF e R) ou efedrina (C-E, CAF-E e R-E) e realizarem os testes comportamentais. No 118? foram anestesiados e eutanasiados por exsanguina??o. Foram avaliados: o peso corporal, ganho de peso, consumo de ra??o, ingest?o cal?rica, coeficiente de efici?ncia alimentar, comprimento naso-anal e ?ndice de massa corporal; peso dos ?rg?os e tecido adiposo abdominal; comprimento do f?mur e t?bia, e teor de minerais totais; teores de colesterol total e fra??es, triacilglicerol e glicemia do soro; teor de lip?dios, colesterol total e triacilglicerol do f?gado; e os efeitos no comportamento pelos testes Labirinto em Cruz Elevado (LCE) eCampo Aberto. O grupo CAF demonstrou maior ingest?o cal?rica, configurando em maior CEA e ac?mulo de tecido adiposo abdominal, al?m de uma tend?ncia em aumento do peso e do IMC. Ademais, CAF obteve eleva??o dos n?veis de triacilglicerol plasm?tico e hep?tico, que possivelmente foram fatores respons?veis pelo aumento do tecido adiposo. Ao mesmo tempo, houve uma rela??o ruim entre as fra??es do colesterol plasm?tico (HDL-c, LDL-c e VLDL-c), com desenvolvimento de dislipidemia. Portanto, a dieta de cafeteria foi capaz de reproduzir um modelo de obesidade humana e de s?ndrome metab?lica em CAF. Os animais de dieta de restri??o obtiveram menor peso corporal e dos ?rg?os, apresentando ainda retardo no crescimento (menor CNA, da t?bia e do f?mur), e menores valores de IMC. O quadro nutricional desse grupo de animais indica que foi poss?vel desenvolver um modelo de desnutri??o. O grupo CAF obteve maior n?mero de entradas nos bra?os fechados do LCE e uma tend?ncia em atravessar maior n?mero de quadrantes no in?cio do teste de Campo Aberto, sugerindoaumento da locomo??o. A efedrina proporcionou: maior n?mero de entradas nos bra?os fechados; de entradas e tempo de perman?ncia nos bra?os abertos do LCE; maior n?mero de entradas, tempo de perman?ncia no centro do e n?mero de quadrantes atravessados no campo aberto; para ambos os tratamentos (CAF-E e R-E), sugerindo um efeito ansiol?tico e de aumento da locomo??o. As altera??es no comportamento frente ? efedrina indicam que os animais CAF e R possuem uma altera??o no sistema dopamin?rgico, que culmina com aumento do efeito ansiol?tico e de locomo??o da droga. / Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, [2016]. / The objective of this work was to evaluate the effects of ephedrine on the behavior of rats treated with standard (commercial) chow, cafeteria diet or caloric restriction diet since lactation. Twelve litters of Wistar rats (Rattus novergicus) were used. The animals were housed in individual cages under standard conditions (natural humidity, temperature of 23 ? C ? 2 and light / dark cycle of 12 hours). The male rats of each litter formed, from lactation to adulthood, the following groups: Control (C) - received standard chow and water ad libitum (n = 24); Cafeteria (CAF) - received cafeteria diet and water ad libitum (n = 24); Restriction (R) - received 50% of the chow consumed by the Control group and water ad libitum (n = 24). Between the 113th and 117th days of life, the animals were subdivided (n = 12) to receive treatment with saline (C, CAF and R) or ephedrine (C-E, CAF-E and R-E) and perform behavioral tests. At 118th, they were anesthetized and euthanized by exsanguination. Body weight, weight gain, feed intake, caloric intake, food efficiency coefficient, naso-anal length and body mass index; weight of organs and abdominal adipose tissue; Length of femur and tibia, and total mineral content; levels of total cholesterol and fractions, triacylglycerol and serum glycemia; lipid content, total cholesterol and triacylglycerol of the liver; and effects on behavior through the Elevated Plus Maze (EPM) and Open Field tests were evaluated. The CAF group demonstrated a higher caloric intake, resulting in an increased CEA and abdominal adipose tissue accumulation, in addition to an increasing tendency of weight and BMI. In addition, CAF obtained an elevation in plasma and hepatic triacylglycerol levels, which were possibly responsible for the increase in adipose tissue. At the same time, there was a great relationship between fractions of plasma cholesterol (HDL-c, LDL-c and VLDL-c), with development of dyslipidemia. Therefore, the cafeteria diet was able to reproduce a model of human obesity and metabolic syndrome in CAF. Restriction diet animals had lower body and organ weight, with slower growth (lower CNA, tibia and femur), and lower BMI values. The nutritional picture of this group of animals indicates that it was possible to develop a model of malnutrition. The CAF group had a higher number of entries in the closed arms of the EPM and a tendency to cross more quadrants at the beginning of the Open Field test, suggesting an increase in locomotion. Ephedrine provided: high number of entries in the closed arms; entries and length of stay in the open arms of the EPM; high number of entries, length of stay in the center and number of quadrants crossed in the open field; for both treatments (CAF-E and R-E), suggesting an anxiolytic effect and increased locomotion. Changes in the behavior against ephedrine indicate that the CAF and R animals have a change in the dopaminergic system, which culminates with aniological increase and locomotion of the drug.
| Identifer | oai:union.ndltd.org:IBICT/oai:acervo.ufvjm.edu.br/jspui:1/1369 |
| Date | January 2016 |
| Creators | Gomes, Arthur Rocha |
| Contributors | Seixas, Sergio Ricardo Stuckert, Riul, Tania Regina, Santos, Antonio Sousa, Leite, H?rcules Ribeiro, Riul, Tania Regina, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Seixas, Sergio Ricardo Stuckert |
| Publisher | UFVJM |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFVJM, instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri, instacron:UFVJM |
| Rights | A concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data., info:eu-repo/semantics/openAccess |
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