Abstract
The effects of dietary sucrose feeding, intragastric sucrose feeding
and dietary calcium-deficiency on primary dentinogenesis of the
pulp-dentin organ and caries progression were examined in an experimental
rat model. The possible role of calcium balance and reduced mineralization
of dentin organic matrix as a cause of reduced dentin formation in young,
fast growing, rats were also studied. During 3-6 weeks of feeding,
immediately after weaning at 3 weeks the urinary calcium, phosphorus,
potassium and sodium levels and excretion rates were determined. The areas
of dentin formed, the width of the predentin layer, serum mineral and
insulin levels, and the areas of dentinal caries lesions were quantified
at the end of the experiment. Also the occurrence and progression level of
caries lesions were measured. In rat pups, dietary sucrose reduced dentin
formation both during the lactation and experimental periods, increased
urinary Ca2+ excretions, reduced urinary P, K
and Na excretions, and enhanced dental caries occurrence and progression,
but it did not affect the width of the predentin layer or the serum
mineral and insulin levels. Intragastric sucrose reduced dentin formation
and increased Ca2+ excretion, but did not
affect the width of the predentin layer, serum mineral and insulin levels
in the blood or induce dental caries. Dietary calcium-deficiency reduced
dentin formation, increased the width of the predentin layer, caused
hypocalcemia, and reduced urinary Ca2+
excretion. These results show that sucrose and calcium-deficiency reduce
the rate of primary dentinogenesis through different mechanisms. Calcium
imbalance or reduced mineralization of the dentin organic matrix does not
explain the reduced dentinogenesis in sucrose fed rats. In conclusion, the
present findings indicate that a sucrose load reduces dentinogenesis by
impairing the synthesis rate of the dentin organic matrix, but also points
out the importance of the local sucrose challenge in initiating dental
caries.
| Identifer | oai:union.ndltd.org:oulo.fi/oai:oulu.fi:isbn951-42-7035-5 |
| Date | 14 June 2003 |
| Creators | Pekkala, E. (Esa) |
| Publisher | University of Oulu |
| Source Sets | University of Oulu |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis, info:eu-repo/semantics/publishedVersion |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess, © University of Oulu, 2003 |
| Relation | info:eu-repo/semantics/altIdentifier/pissn/0355-3221, info:eu-repo/semantics/altIdentifier/eissn/1796-2234 |
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