Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Student: Vendula Damborská Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: The Optimization of the growth of the plant tissue culture of Juniperus virginiana The main goal of this diploma thesis lies in the optimization of cultivation medium composition for the tissue culture of Juniperus virginiana (varieties 'Hetzii', 'Glauca', 'Grey Owl'). The best results of all tested media were achieved by using Schenk and Hildebrandt medium with an addition of α-NAA (3.0 mg.l-1 ), kinetin (0.2 mg.l-1 ) and ascorbic acid (15.0 mg.l-1 ). The cultures were cultivated at the temperature of 25 řC and light period of 16 hours light/8 hours dark. The growth curve for the tissue culture of Juniperus virginiana 'Glauca' was set for 2 different cases: with and without the addition of phenylalanin (biogenetic precursor of phenylpropans biosynthesis). The maximal growth was reached on 35th day of the cultivation in both cases, phenylalanin increases the growth rate by 29.65 %.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:331245 |
| Date | January 2015 |
| Creators | Damborská, Vendula |
| Contributors | Kašparová, Marie, Siatka, Tomáš |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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