This project was conducted to further elucidate the role of matrix metalloproteinase 7 (MMP7) in the pathogenesis of the carcinogenic bacteria Helicobacter pylori. Specifically, in this thesis I discuss the role of MMP7 in H. pylori-induced inflammation and injury. Briefly, we performed histological and molecular biological analysis of stomach tissue from wild-type and MMP7-/- mice that had been challenged with H. pylori. We also performed molecular biological analyses of macrophages extracted from the stomachs or derived from bone marrow of wild-type and MMP7-/- mice. We found that loss of MMP7 results in increased production of inflammatory cytokines, and that MMP-/- macrophages display enhanced M1 macrophage phenotypes. In this research we discovered an explanation for the increased inflammation and injury seen in MMP7-/- mice and thus, a potential role for MMP7 in protecting wild-type animals from H. pylori- induced inflammation.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-10302012-135509 |
| Date | 31 October 2012 |
| Creators | Krakowiak, Michelle Stokes |
| Contributors | Richard Peek |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-10302012-135509/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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