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Previous issue date: 2004-02-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The utilization of the immunohistochemical/histochemical double staining technique comes permitting the analysis of two molecular parameters at the same time in an even tissue. Starting from this, the objective of this study was to investigate the existence of differences between the number of NORS/NUCLEUS between p53 positive and p53 negative cells, as well as the existence of correlation between the medium of the NORs of the p53 positive and negative cells and the histological scores of maligninancy in 16 cases of oral squamous cell carcinoma. It was first classified in agreement with the histological grade system of maligninancy proposed by Bryne (1998) and the double staining technique immunohistochemistry/histochemistry was utilized for the achievement to quantify of the NORs in p53 positive and negative cells. It had not significant differences between the medium number of NORs of the p53 positive cells and of the p53 negative cells, and they were not correlated with the histological scores of malignancy. We conclude that the related phenotype to the p53 immunohistochemical expression did not influence the average of NORS/NUCLEUS and this medium, in both positive and negative cells, is not correlated with the degree of histological aggressivity of the oral squamous cell carcinoma / A utiliza??o da t?cnica de dupla marca??o imuno-histoqu?mica /histoqu?mica vem permitindo a an?lise de dois par?metros moleculares ao mesmo tempo em um mesmo tecido. Partindo deste princ?pio, o objetivo deste estudo foi pesquisar em carcinoma epiderm?ide oral o n?mero m?dio de NORs/n?cleo entre c?lulas p53 positivas e p53 negativas por interm?dio da dupla marca??o imuno-histoqu?mica/histoqu?mica, bem como a correla??o do n?mero m?dio de NORs/n?cleo das c?lulas p53 positivas e negativas com os escores histol?gicos de malignidade. Foram estudados 16 casos de carcinoma epiderm?ide, que foram classificados de acordo com o sistema de grada??o histol?gica de malignidade proposto por Bryne (1998). A t?cnica de dupla marca??o imuno-histoqu?mica/histoqu?mica foi utilizada para a realiza??o da contagem das NORs em c?lulas p53 positivas e negativas. N?o houve diferen?a estatisticamente significante entre o n?mero m?dio de NORs/n?cleo das c?lulas p53 positivas e negativas. Tamb?m n?o houve correla??o entre o n?mero m?dio de NORs/n?cleo das c?lulas p53 positivas e negativas e os escores histol?gicos de malignidade. Conclu?mos, ent?o, que o fen?tipo relacionado ? express?o imuno-histoqu?mica do p53 n?o influi na m?dia de NORs/n?cleo e esta m?dia, tantos das c?lulas positivas quanto das negativas, n?o se correlaciona com o grau de agressividade histol?gica do carcinoma epiderm?ide oral
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17138 |
| Date | 17 February 2004 |
| Creators | Melo, Francielli Cristine Cunha |
| Contributors | CPF:32381611487, http://lattes.cnpq.br/7688226005563390, Freitas, Roseana de Almeida, CPF:28444361453, http://lattes.cnpq.br/9512014003639405, Costa, Ant?nio de Lisboa Lopes |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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