Return to search

Studies on the immunomodulatory and anti-tumour activities of green tea catechins.

by Tung Kai Chiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 152-166). / Abstracts in English and Chinese. / STATEMENT --- p.i / ACKNOWLEDGEMENTS --- p.ii / ABBREVIATIONS --- p.iv / ABSTRACT --- p.vii / 撮要 --- p.x / TABLE OF CONTENTS --- p.xiii / Chapter CHAPTER 1: --- GENERAL INTRODUCTION / Chapter 1.1 --- AN OVERVIEW OF THE IMMUNE SYSTEM --- p.1 / Chapter 1.1.1 --- Innate Immunity --- p.1 / Chapter 1.1.2 --- Specific Acquired Immunity --- p.4 / Chapter 1.2 --- IMMUNE RESPONSE TO TUMOURS --- p.7 / Chapter 1.2.1 --- Specific Mechanisms --- p.7 / Chapter 1.2.1.1 --- Cellular Immune Response --- p.7 / Chapter 1.2.1.2 --- Humoral Immune Response --- p.9 / Chapter 1.2.2 --- Non-specific Mechanism --- p.10 / Chapter 1.2.2.1 --- Natural Killer Cells --- p.10 / Chapter 1.2.2.2 --- Lymphokine-activated Cells --- p.11 / Chapter 1.2.2.3 --- Tumour Infiltrating Lymphocytes --- p.12 / Chapter 1.2.2.4 --- Macrophages --- p.13 / Chapter 1.2.2.5 --- Other Cell Types Mediating Non-specific Immunity --- p.14 / Chapter 1.3 --- PHYTOCHEMICALS AS POTENTIAL IMMUNOMODULATORS and anti-tumour agents --- p.15 / Chapter 1.3.1 --- Modulation of the Immune System --- p.15 / Chapter 1.3.2 --- Induction of Cancer Cell Differentiation --- p.16 / Chapter 1.3.3 --- Stimulation of Apoptosis --- p.17 / Chapter 1.3.4 --- Other Anti-tumour Mechanisms --- p.18 / Chapter 1.4 --- GREEN TEA: GENERAL PROPERTIES AND PHARMACO- logical activities --- p.19 / Chapter 1.4.1 --- General Introduction --- p.19 / Chapter 1.4.2 --- Chemistry of Tea --- p.21 / Chapter 1.4.3 --- Physiological and Pharmacological Effects of Green Tea Catechins --- p.26 / Chapter 1.4.3.1 --- Anti-oxidative Activity --- p.26 / Chapter 1.4.3.2 --- Anti-mutagenic Activity --- p.27 / Chapter 1.4.3.3 --- Anti-carcinogenic Activity --- p.27 / Chapter 1.4.3.4 --- Anti-inflammatory Activity --- p.32 / Chapter 1.4.3.5 --- Hypocholesterolemic and Hypolipidemic Activities --- p.32 / Chapter 1.4.3.6 --- Anti-microbial Activity --- p.33 / Chapter 1.5 --- aims and scopes of this investigation --- p.34 / Chapter CHAPTER 2: --- MATERIALS AND METHODS / Chapter 2.1 --- MATERIALS --- p.36 / Chapter 2.1.1 --- Animals --- p.36 / Chapter 2.1.2 --- Cell lines --- p.36 / Chapter 2.1.3 --- Green Tea Catechins and Epicatechin Isomers --- p.38 / Chapter 2.1.4 --- Recombinant Murine Interleukin-2 (rmIL-2) --- p.38 / Chapter 2.1.5 --- Antibodies --- p.39 / Chapter 2.1.6 --- "Buffers, Culture Medium and Other Reagents" --- p.40 / Chapter 2.1.7 --- Reagents for Gel Electrophoresis --- p.47 / Chapter 2.1.8 --- Radioisotopes --- p.48 / Chapter 2.2 --- METHODS --- p.49 / Chapter 2.2.1 --- "Isolation, Purification and Characterization of Green Tea Catechins and Epicatechin Isomers" --- p.49 / Chapter 2.2.1.1 --- Extraction of Green Tea Catechins --- p.49 / Chapter 2.2.1.2 --- Analysis of Epicatechin Isomers by HPLC --- p.49 / Chapter 2.2.1.3 --- Determination of the Bio-toxicity of Green Tea Catechins --- p.50 / Chapter 2.2.1.4 --- Assay of In Vitro Cytotoxicity of Green Tea Catechins on Splenocytes --- p.51 / Chapter 2.2.2 --- Assays for the Immunomodulatory Activities of Green Tea Catechins --- p.52 / Chapter 2.2.2.1 --- Isolation and Preparation of Cells --- p.52 / Chapter 2.2.2.2 --- In Vitro Lymphocyte Transformation Assay --- p.53 / Chapter 2.2.2.3 --- Mixed Lymphocyte Culture --- p.53 / Chapter 2.2.2.4 --- Colorimetric Assay of Alloreactive Cytotoxic T Lymphocytes --- p.54 / Chapter 2.2.2.5 --- Foodpad Swelling Assay of Delayed-type Hypersensitivity --- p.55 / Chapter 2.2.2.6 --- Haemagglutination Assay of In Vivo Antibody Formation --- p.56 / Chapter 2.2.2.7 --- Characterization of the Lymphocyte Subpopulations from Spleen by Flow Cytometry --- p.56 / Chapter 2.2.2.8 --- In Vivo Migration of Macrophages --- p.57 / Chapter 2.2.2.9 --- In Vitro Assay of Phagocytic Activity of PEC --- p.58 / Chapter 2.2.2.10 --- In Vitro Assay of Macrophage-mediated Cytostatic Activity --- p.58 / Chapter 2.2.3 --- Assays for the Anti-tumour Activities of Green Tea Catechins --- p.60 / Chapter 2.2.3.1 --- Assay of In Vivo Anti-tumour Activity --- p.60 / Chapter 2.2.3.2 --- Induction and Assay of Natural Killer Cell Activity --- p.60 / Chapter 2.2.3.3 --- Induction and Assay of Lymphokine-activated Killer Cell Activity --- p.61 / Chapter 2.2.3.4 --- Assay of In Vitro Tumour Cell Proliferation --- p.62 / Chapter 2.2.3.5 --- In Vitro Tumour Clonogenicity Assay --- p.63 / Chapter 2.2.3.6 --- Induction of Myeloid Leukemic Cell Differentiation --- p.63 / Chapter 2.2.3.7 --- DNA Fragmentation Analysis --- p.64 / Chapter 2.2.3.8 --- Cell Cycle and DNA Content Evaluation --- p.66 / Chapter 2.2.4 --- Statistical Analysis --- p.66 / Chapter CHAPTER 3: --- "Extraction, Purification and Characterization of GTCs" / Chapter 3.1 --- INTRODUCTION --- p.67 / Chapter 3.2 --- RESULTS --- p.69 / Chapter 3.2.1 --- "Extraction of Catechins from the Chinese Green Tea, Ji Pin Long Jing" --- p.69 / Chapter 3.2.2 --- Analysis of Epicatechin Isomers by HPLC --- p.69 / Chapter 3.2.3 --- Bio-toxicity Determination by Brine Shrimp Bioassay --- p.71 / Chapter 3.2.4 --- Effect of GTCs on the Viability of Murine Splenocytes --- p.72 / Chapter 3.3 --- DISCUSSION --- p.74 / Chapter CHAPTER 4: --- The Immunomodulatory Activities of GTCs / Chapter 4.1 --- INTRODUCTION --- p.75 / Chapter 4.2 --- RESULTS --- p.77 / Chapter 4.2.1 --- Effects of GTCs on the In Vitro Proliferation of Murine Splenocytes --- p.77 / Chapter 4.2.2 --- In Vitro Co-mitogenic Effect of GTCs on Murine Splenocytes --- p.77 / Chapter 4.2.3 --- Stimulation of Lymphocyte Proliferation In Vitro by Intraperitoneal Administration of GTCs --- p.81 / Chapter 4.2.4 --- Effect of In Vivo Treatment of GTCs on In Vitro Mixed Lymphocyte Reaction --- p.81 / Chapter 4.2.5 --- Effect of In Vivo Administration of GTCs on Splenic Lymphocytes Subpopulations --- p.87 / Chapter 4.2.6 --- Effect of In Vivo Administration of GTCs on the Induction of Alloreactive Cytotoxic T Lymphocytes --- p.87 / Chapter 4.2.7 --- In Vivo Induction of Delayed-type Hypersensitivity (DTH) Response to Sheep Red Blood Cells (SRBC) by GTCs --- p.91 / Chapter 4.2.8 --- Primary Humoral Immune Response to SRBC in GTCs- treated Mice --- p.91 / Chapter 4.2.9 --- Effect of GTCs on In Vivo Migration of Macrophages --- p.95 / Chapter 4.2.10 --- Effect of GTCs on the Phagocytic Activity of Thioglycollate- elicited Macrophages In Vitro --- p.95 / Chapter 4.2.11 --- Effect of In Vivo Administration of GTCs on Phagocytic Activity of Thioglycollate-elicited Macrophages In Vitro --- p.98 / Chapter 4.2.12 --- Effect of GTCs on In Vitro Cytostatic Activity of Picolinic Acid-activated Macrophages --- p.98 / Chapter 4.2.13 --- Effect of In Vivo Administration of GTCs on the Cytostatic Activity of Picolinic Acid-activated Macrophages --- p.101 / Chapter 4.3 --- DISCUSSION --- p.103 / Chapter CHAPTER 5: --- THE ANTI-TUMOUR ACTIVITIES OF GTCs / Chapter 5.1 --- introduction --- p.107 / Chapter 5.2 --- RESULTS --- p.109 / Chapter 5.2.1 --- Effects of GTCs on the Growth of Transplantable Tumour Cells In Vivo --- p.109 / Chapter 5.2.2 --- Effect of GTCs on In Vivo Induction of Natural Killer Cells --- p.109 / Chapter 5.2.3 --- Effect of GTCs on In Vitro Induction of Lymphokine- activated Killer Cell Activity --- p.113 / Chapter 5.2.4 --- In Vitro Cytotoxicity of Green Tea Epicatechin Isomers on Murine and Human Tumour Cell Lines --- p.115 / Chapter 5.2.5 --- In Vitro Cytostatic Effect of EGCG on Various Tumour Cell Lines --- p.118 / Chapter 5.2.6 --- Effect of EGCG on the In Vitro Clonogenicity of Myeloid Leukemia Cells --- p.127 / Chapter 5.2.7 --- Induction of Apoptosis of Myeloid Leukemia HL-60 Cells In Vitro --- p.130 / Chapter 5.2.8 --- Effect of EGCG on the Cell Cycle Kinetics of Myeloid Leukemia HL-60 Cells In Vitro --- p.131 / Chapter 5.2.9 --- Effect of EGCG on the Morphological Changes of Myeloid Leukemia Cells --- p.136 / Chapter 5.2.10 --- Effect of EGCG on the Endocytic Activity of Myeloid Leukemia Cells --- p.136 / Chapter 5.3 --- discussion --- p.141 / Chapter CHAPTER 6: --- CONCLUSIONS AND FUTURE PERSPEC- TIVES --- p.145 / REFERENCES --- p.152

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322800
Date January 1999
ContributorsTung, Kai Chiu., Chinese University of Hong Kong Graduate School. Division of Biochemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xviii, 166 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Page generated in 0.0033 seconds